In cell and gene therapy manufacturing, protecting cell health is critical at every step. Fluidhandling systems must move cells reliably and efficiently—without introducing unnecessary stress that could impact viability or function.
The iFILL™ Micro Hybrid Piston Diaphragm (HPD) pump was developed with this challenge in mind. Unlike traditional peristaltic pumps that rely on repeated tubing compression, the iFILL™ Micro uses a precision controlled diaphragm inside a disposable fluid path. This approach maintains a closed, sterile system while eliminating continuous tubing deformation—offering a gentler way to move sensitive cells.
To ensure this design performs where it matters most, Intellitech evaluated the iFILL™ Micro in a direct comparison study using primary human T cells, a clinically relevant and highly delicate cell type commonly used in cell therapy workflows. Cells were circulated through closed systems using either the iFILL™ Micro or a standard peristaltic pump under equivalent operating conditions.
The Results: Cell Health Preserved
The study showed that cells pumped with the iFILL™ Micro remain healthy and functional, performing on par with cells handled by traditional peristaltic pumps:
- High cell viability (>97%) was maintained across all test conditions
- No delayed or hidden damage was observed over a sevenday recovery period
- Normal cell morphology and integrity were preserved throughout
In short, the iFILL™ Micro demonstrated that it can safely handle fragile human T cells without compromising viability, recovery, or longer term cellular performance.
Why It Matters
These findings give manufacturers confidence that the iFILL™ Micro can be integrated into existing and nextgeneration cell therapy processes without introducing additional risk to valuable cell products. At the same time, its unique pumping mechanism offers meaningful advantages beyond cell safety, including:
- Precise, repeatable fluid control
- Reduced mechanical wear on disposable components
- A robust alternative to traditional peristaltic pumping
A Modern Option for Advanced Cell Manufacturing
By combining proven cell viability with a differentiated fluidhandling approach, the iFILL™ Micro pump provides a reliable, forwardlooking solution for advanced cell and gene therapy workflows—supporting both today’s manufacturing needs and tomorrow’s innovations.
